Influence of Different Anticoagulant Tube on Measurement Results of Homocysteine
- VernacularTitle:不同抗凝管对同型半胱氨酸测定结果的影响
- Author:
Jin HE
;
Qin YAO
;
Lujie WANG
;
Jun ZHANG
- Publication Type:Journal Article
- Keywords:
Homocysteine;
High-performance liquid chromatography;
Stability
- From:
Journal of Kunming Medical University
2013;(11):18-20
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of different anticoagulant tube of blood for blood plasma total homocysteine (tHcy) . Methods Six healthy subjects were selected, then the fasting venous blood samples were collected with EDTA-2k tube,sodium citrate tube and lithium heparin tube, respectively. The blood samples were kept under different conditions. The concentrations of tHcy were determined by quantitative HPLC method and the data were analyzed using SPSS17.0 software. Results Under the conditions of 4℃placed for 2 h, 4 h, 7 h, and- 20 ℃ placed for 1 d, 3 d, 5 d after separating plasma, the concentrations of plasma tHcy in different anticoagulant tubes were no statistical difference. In addition, after placing the whole blood at 4℃for 2 h, 4 h, 7 h, the concentrations of plasma tHcy of separated plasma in different anticoagulant tubes were no statistical differences. Conclusion There are no significant differences in determination of the concentration of plasma tHcy when using EDTA-2k tube,sodium citrate tube and lithium heparin tube sampling blood.
