Reteplase fusion protein：expression, purification and the effect of chaperones on its renaturation
- VernacularTitle:瑞替普酶融合蛋白的表达、纯化及分子伴侣对其复性的影响
- Author:
Wei WANG
;
Wenyi ZOU
;
Qinglin FAN
;
Lihua SONG
- Publication Type:Journal Article
- Keywords:
reteplase;
fusion protein;
chaperones;
renaturation
- From:
Acta Universitatis Medicinalis Anhui
2013;(11):1287-1291
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the prokaryotic expression system of reteplase fusion protein and research the effect of chaperones on its renaturation. Methods Inserted the reteplase gene into the prokaryotie expression vector PET32a and then expressed it by the induction of IPTG in E. coli BL21. Researched the effect of chaperones on the renaturation of fusion protein by adding different chaperones. Results The analysis of SDS-PAGE and Western blot indicated that reteplase fusion protein was expressed correctly. Chaperones DsbA,pKJE7,pTf16 had the conspicu-ous effect on the renaturation of fusion protein. The result of activity assay indicated that the refolded reteplase fu-sion protein had fibrinolytic activity. Conclusion Chaperones can promote renaturation of reteplase fusion protein.
