HPLC Determination of Three Kinds of Flavonoids Content in Flowers of Dimocarpus Longan Lour

VernacularTitle:HPLC法同时测定龙眼花中3种黄酮类化合物含量
Author: Jie LIANG ; Jing YU ; Xianfu LIU ; Zhengyi SUN
Publication Type:Journal Article
Keywords: Flowers of Dimocarpus longan Lour.; quercetin; luteolin; kaempferol; HPLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(7):1576-1580
CountryChina
Language:Chinese
Abstract: This study was aimed to create a method for content determination of quercetin, luteolin and kaempferol in flowers of Dimocarpus longan Lour. from different habitats of Guangxi province by HPLC. The samples were sepa-rated on the Hypersil C18 column (250 mm í 4.6 mm, 5 μm) which was eluted with methanol-0.2% phosphoric acid solution (47:53) with detective wavelength at 360 nm, flow rate at 1.0 mL·min-1, and column temperature at 30℃. The results showed that this method had a good linear relationship within the range of 0.18 to 2.88 μg for quercetin, 0.059 to 0.944 μg for luteolin, and 0.024 to 0.384 μg for kaempferol (r = 0.9999). The average recovery rate was 100.06% (RSD = 1.72%), 99.77% (RSD = 1.18%) and 98.67% (RSD = 1.99%, n = 9), respectively. It was conclud-ed that this method is simple, rapid, reliable and with good repeatability, which can be used in the quality control of flowers of Dimocarpus longan Lour.