The study of secretory clusterin on the biological behaviors of non-small cell lung cancer cells
10.3969/j.issn.1007-3969.2013.09.001
- VernacularTitle:分泌型簇集素蛋白在肺癌细胞株中生物学行为的研究
- Author:
Xiao MA
;
Xiaoying LUO
;
Zonghai LI
;
Hecheng LI
- Publication Type:Journal Article
- Keywords:
Secretory clusterin;
Non-small cell lung cancer;
Cell migration;
MicroRNA
- From:
China Oncology
2013;(9):697-702
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: Primary study showed that clusterin was associated with tumorigenicity. The goal of the study is to investigate the role of secretory clusterin in non-small cell lung cancer cell A549/H460. Methods: The lung cancer cell A549/H460 was treated with purified secretory clusterin, the Boyden Chamber migration assay was used to detected the migration of the lung cancer cell;the CCK8 assay was used to detected the growth of the cells;microRNA expression spectrum in H460 treated with secretory clusterin was analyzed, after that, we used the real-time florescence quantification detected the expression of microRNA in H460, and the biological function of microRNA molecular mechanisms of secretory clusterin was analyzed. Results: Secretory clusterin promoted the migration in A549/H460 (P<0.000 1);Secretory clusterin inhibited the growth in H460/A549 (P<0.000 1);MicroRNA-302b-3p, microRNA-23a-5p and microRNA-101-5p was overexpressed in H460 when treated with secretory clusterin. Conclusion:Secretory clusterin could promote the migration and inhibit the growth of lung cancer cell;It could change the microRNA expression spectrum as well. Our studies revealed that secretory clusterin could be used as a tool for further study, and it is a potential target in the treatment of lung cancer.
