Down-regulation of microRNA-187*expression in colorectal cancer and its roles in promoting cell ;apoptosis
10.3969/j.issn.1007-3969.2013.09.002
- VernacularTitle:微小RNA-187*在结直肠癌组织中表达及其对结肠癌细胞凋亡的影响
- Author:
Bo LIU
;
Yanfeng TIAN
;
Zengren ZHAO
;
Zhibin FAN
;
Lijing ZHANG
;
Xinqi HE
;
Lifei GAO
- Publication Type:Journal Article
- Keywords:
Colorectal cancer;
microRNA;
microRNA-187*;
Apoptosis
- From:
China Oncology
2013;(9):703-708
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: MicroRNAs (miRNAs) play an important role in tumor biological behavior. miRNAs are down-regulated or up-regulated in various cancer types, triggering abnormal cell differentiation, proliferation and apoptosis. This study was designed to investigate the expression and clinical signiifcance of miR-187*in colorectal cancer (CRC), and further to investigate its roles in promoting cell apoptosis. Methods:The expressions of miR-187* in 40 CRC cases were examined by real-time quantitative reverse transcription-PCR (qRT-PCR). The relationship between miR-187*expression and clinical features of CRC was analyzed. HCT116 cells were transfected with a miR-187*mimic and the apoptosis of the transfected cells were examined by lfow cytometry (FCM). Results:The expression of miR-187*was down-regulated in CRC tissues 0.165 (0.106, 0.428) compared with those in normal tissues 0.334 (0.211, 0.712) (P<0.05), especially in mucinous carcinoma and older age CRC (P<0.05). Transfection of HCT116 cells with a miR-187*mimic up-regulated the expression of miR-187*and increased cell early apoptosis (P<0.05). Conclusion: The expression level of miR-187* was lower in CRC. miR-187* expression correlates with histological type and age. Transfection of HCT116 cells with a miR-187*mimic accelerates apoptosis of tumor cells, suggesting that miR-187*is a potent tumor suppressor.
