Salvia miltiorrhiza monomer IH764-3 protects human brain microvascular endothelial cell from hypoxia/reoxygention injury through inhibiting leukocyte activity
- VernacularTitle:丹参单体IH764-3通过抑制白细胞激活产物减轻人脑微血管内皮细胞的缺氧-复氧损伤
- Author:
Ran ZHOU
;
Wenjian ZHANG
;
Jinning LOU
;
Liya YE
;
Chenghui LI
- Publication Type:Journal Article
- Keywords:
salvia miltiorrhiza monomer IH764-3;
leukocyte;
brain;
endothelium;
hypoxia-reoxygenation injury
- From:
Basic & Clinical Medicine
2010;30(1):28-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of IH764-3 on the leukocyte-mediated hypoxia-reoxygention injury of human brain microvascular endothelial cell (HBM VEC). Methods MTT assay was used to detect the survival of HBMVEC; gelatin zymography was used to check the activity of MMPs. The level of reactive oxygen species (ROS) in leukocyte was determined via commercially available kit, and the indirect enzyme-linked immunosorbent assay (ELISA) was used to quantify the contents of TNF-α, IL-1α, IL-2 and INF-γ in leukocyte culture medium. Results Survival of HBMVEC was impaired by hypoxia-reoxygenation, which was aggravated by supernatant of activated leukocytes but was attenuated by IH764-3. Leukocytes produced high level of MMP-9, ROS and cytokines (TNF-α, IL-1α, IL-2, IFN-γ) after hypoxia-reoxygenation, the process was inhibited by IH 764-3. Furthermore, IH764-3 could effectively reverse hypoxia-reoxygenation injury of HBMVEC with supernatant of activated leukocytes. Conclusion IH764-3 can protect HBMVEC from leukocyte-involved hypoxia-reoxygenation injury by attenuating the activation of leukocytes and inhibiting the pathogenic effects of leukocytes products.
