Detection of systematic oxidative stress in preeclampsia
- VernacularTitle:子痫前期患者系统性氧化应激反应的检测
- Author:
Zhongqing QIAN
;
Yaoying ZENG
;
Bin ZHU
;
Yuhua JI
;
Fang HE
- Publication Type:Journal Article
- Keywords:
preeclampsia;
oxidative stress;
placenta;
polymorphonuclear neutrophil
- From:
Basic & Clinical Medicine
2010;30(4):343-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect systematic oxidative stress in preeclampsia.Methods (1)Morphological features of placenta hypoxia were observed by histological method ; (2) Level of granulocyte intracellular reactive oxygen species was monitored by dyeing full blood with 2' ,7'-dichlorodihydrofluorescein diacetate (H2DCFDA) ; (3) Level of H_2O_2 in sera was detected by special kits.Results Compared to normal pregnancy,placentas from preeclampsia showed distinct features of hypoxic stress injury,such as more syncytial knots formation,fibrosis emerged,vein in-jury and loss its normal configuration; Fluorescence values of ROS probe in neutrophils from different women were 45.61±12.20(n =49),51.02 ± 13.60(n =56,P <0.01)and 85.10 ± 16.30(n =47,P <0.01); Concentra-tions of H_2O_2were (24.57±5.17)μmol/L(n =49),(26.61±3.25)μmol/L(n =56,P 0.01) and (39.84±9.67)μmol/L(n=47,P<0.01) respectively.Conclusion With the help of histological method,flow cytometry and special kits,systematic oxidative stress can be detected through checking placentic tissues,netrophils and sera of preeclampsia.
