Effects of repeated hypoxic exposures on glycolysis, mitochondrial oxidative phosphorylation and energy charge in mouse brain
- VernacularTitle:急性重复低氧对小鼠脑组织中糖酵解和线粒体氧化磷酸化以及能量负荷的影响
- Author:
Xin LI
;
Shengnan XIE
;
Juanjuan YIN
;
Guangwei LIU
;
Yaohua LI
;
Guowei Lü
;
Shun YU
- Publication Type:Journal Article
- Keywords:
hypoxia;
phosphofructokinase;
pyruvate kinase;
mitochondrial complex Ⅰ;
energy charge
- From:
Basic & Clinical Medicine
2009;29(12):1254-1257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of repeated hypoxic exposures (HEs) on glycolysis, mitochondrial oxi-dative phosphorylation and energy charge in mouse brain. Methods Adult BALB/c mice were repeatedly exposed to hypoxia for 5 times and the standard tolerant time and body temperature were recorded. The activities of PFK, PK and mitochondrial complex Ⅰ in the brain were assayed. Phosphoadenosines and energy charge were measured. Results Repeated HEs prolonged the hypoxic tolerance and reduced the body temperature. The activities of PFK and PK experienced regular changes, with an increase in 1st and 3rd HEs and a decline to control levels in 5th HE. The complex Ⅰ activity continued to decrease during HEs. The energy charge was stable. Conclusion HEs lead to a regular change of glycolysis, a continued inhibition of mitochondrial oxidative phosphorylation, and a main-tained energy charge in the brains of mouse.
