Optimization of recombinant human endostatin refolding
- VernacularTitle:大肠杆菌表达的新一代重组人内皮抑素复性条件的优化
- Author:
Lixi ZHAO
;
Yongping JIANG
- Publication Type:Journal Article
- Keywords:
recombinant human endostatin;
refolding;
purification;
chick embryo chorioallantoic membrane assay
- From:
Basic & Clinical Medicine
2010;30(5):492-495
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the conditions for enhancing the refolding of a novel recombinant human(rh)endostatin and test the biological activities of refolded endostatin.Methods The partial purified inclusion bodies of rh-endostatin were dissolved with 6 mol/L guanidine-HCl followed by combination of dilution and dialysis of the dissolved endostatin.The refolded endostatin was then purified by cation-exchange chromatography.The biological activities of purified rh-endostatin were assessed by endostatin-specific monoclonal antibody and chick embryo chorioallantoic membrane assay.Results A 46% refolding yield was achieved after optimizing the refolding conditions.The purified endostatin reacted with specific anti-endostatin monoclonal antibody and showed significant inhibition of angiogenesis in chick embryo ehorioallantoic membrane assay.Conclusion The method of highest refolding yield of human endostatin was developed.This optimized method significantly promotes the application of this novel human endostatin to preclinical and clinical studies.
