Effect of HPV E6 siRNA on the proliferation and chemotherapy sensitivity of cervical cancer HeLa cells
- VernacularTitle:HPV18 E6 siRNA对宫颈癌细胞增殖及化疗药物敏感性的影响
- Author:
Jinghua LI
;
Weijuan WANG
;
Wanli GAO
;
Lei GUO
;
Limin FENG
- Publication Type:Journal Article
- Keywords:
cervical cancer;
HPV18 E6 siRNA;
paclitaxel liposome;
cell proliferation activity;
chemotherapy sensitivity
- From:
Basic & Clinical Medicine
2010;30(5):500-504
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidates the effects of HPV18 E6 siRNA targeting at human papillomavirus(HPV)18 E6 gene on the proliferative activity of HeLa cells and chemotherapy sensitivity.Methods HPV18 E6 expression of HeLa cells was inhibited by siRNA interference,the change of P53 and P21 proteins expression level was measured by Western blot.MTT assay was used to detected proliferative activity and sensitivity to paclitaxel liposome of HeLa cells.Results After inhibition of E6 expression,P53 and P21 proteins increased and the growth of HeLa cells was decreased(P ＜0.01).The inhibition rate of HeLa was markedly increased after transfection of HPV18 E6 siRNA and paclitaxel liposome.Conclusion HPV18 E6 siRNA can effectively silence gene expression of E6 and inhibit proliferation of HeLa cells.HeLa cells are more sensitive to combine HPV18 E6 siRNA with paclitaxel liposome than that of control groups.
