Acute application of ApoE4 increases the neuronal resting [Ca~(2+)] i in rat cortex
- VernacularTitle:急性给予ApoE4升高大鼠皮层神经元内静息[Ca~(2+)]i
- Author:
Xinai LIU
;
Xinyi LI
- Publication Type:Journal Article
- Keywords:
ApoE;
intracellular calcium;
NMDA;
Fluo-3/AM
- From:
Basic & Clinical Medicine
2010;30(1):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the acute effect of apolipoprotein E(apoE)on the intracellular free Ca~(2+) of rat cortical neurons. Methods The intracellular resting calcium level in cultured primary rat cortical neurons was measured by using confocal fluorescent imaging technique. MK-801, an N-methyl-D-aspartate (NMDA) receptor noncom-petitive antagonist, was employed to test potential function of apoE4 through blocking NMDN receptor. Results Acute application of apoE4, but not apoE3, significantly increased the resting [Ca~(2+)] i in a dose-and time-dependent manner (P <0. 01 or P <0. 05) , and MK-801 partly blocked the apoE4-induced elevation of resting [Ca~(2+)]i (P <0. 05 or P <0. 01). Conclusion Acute administration of ApoE4 disturbs calcium homeostasis, and the activation of NMDA receptor may play a critical role in the intracellular calcium overload induced by apoE4 and neurotoxicity.
