Puerarin reduces ethanol-induced-apoptosis of spermatogenic cells in rat testis
- VernacularTitle:葛根素减轻乙醇导致大鼠生精细胞的凋亡
- Author:
Hongyun HE
;
Yihao DENG
;
Xinwen YANG
;
Kaiming YANG
- Publication Type:Journal Article
- Keywords:
ethanol;
puerarin;
spermatogenie cells;
apoptosis
- From:
Basic & Clinical Medicine
2009;29(12):1291-1295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate potential protection by protect against ethanol-induced apoptosis of spermato-genie cells in rat testis. Methods Thirty SD adult male rats were randomly divided into three groups: normal group, alcohol group and puerarin group. At 40th day, BCL-2 and BAX of spermatogenic cells of testis tissue were checked by RT-PCR and immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. Re-suits The results of RT-PCR and immunohistochemistry indicated that BCL-2 and BAX of spermatogenie cells were not significanty different between puerarin group and normal group, but there was the significant difference between alcohol group and puerarin group (P <0.01). Apoptosis of spermatogenic cells in alcohol group was significantly higher than normal group. Conclusion Spermatogenic cells could generate apoptosis by changing the expression of BCL-2 and BAX. Puerarin could inhibit this damage of didymus by alcohol.
