Lentivirus PARG-shRNA transfection decreases matrix adhesion,migration and invasion potencies of colon carcinoma lovo cells
- VernacularTitle:慢病毒PARG-shRNA转染降低大肠癌lovo细胞基质黏附、运动和侵袭能力
- Author:
Qiaozhuan LI
;
Yalan WANG
;
Xian LI
- Publication Type:Journal Article
- Keywords:
poly(ADP-ribose) glycohydrolase.shRNA;
lentivirus;
colon carcinoma;
invasion
- From:
Basic & Clinical Medicine
2010;30(3):237-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of poly(ADP-ribose) glycohydrolase (PARG) gene silencing on colon carcinoma lovo cells matrix adhesion,migration and invasion.Methods Lentivirus PARG-shRNA was transfected into colon carcinoma lovo cells and the lovo cell strain with PARG gene silencing perpetually was selected.Western blot was used to test the expression of PARG,poly(ADP-ribose) polymerase(PARP) and NF-κB.The lovo cell matrix adhesion,migration and invasion potencies were observed by cell matrix adhesion,migration and invasion assay.Results The expression of PARP and NF-κB in experiment groups was weaker than that in the control groups (P＜0.05).PARG gene silencing of lovo cells decreased the cell matrix adhesion,migration and invasion potencies.The inhibitory rates of lovo cell matrix adhesion,migration and invasion were 25.22%,38.71%,35.29% respectively.Conclusion PARG gene silencing of lovo cells could inhibite cell matrix adhesion,migration and invasion potencies,which may relate to that PARG gene silencing and decreasing the activity of PARP and NF-κB.PARG probably plays an important role in the infiltration and metastasis of tumor.
