Increased expression of ROCK-Ⅰ, p-MBS Thr-697 and α-SMA during hepatic fibrogenesis
- VernacularTitle:大鼠肝纤维化组织内ROCK-Ⅰ、磷酸化MBS Thr-697和α-SMA表达增强
- Author:
Caixia HU
;
Jiasheng ZHENG
;
Yuzhen WANG
;
Huiqing JIANG
- Publication Type:Journal Article
- Keywords:
hepatic fibrosis;
Rho-associated coiled-coil kinase;
cytoskeleton
- From:
Basic & Clinical Medicine
2010;30(4):355-359
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the dynamic expression of ROCK-Ⅰ,p-MBS Thr-697,α-SMA protein and their mRNA in the hepatic fibrogenesis and the changes of actin cytoskeleton.Methods ROCK-Ⅰ and p-MBS Thr-697 protein in liver were determined by Western blot and their mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR),while the distribution of ROCK-Ⅰ and α-SMA in liver was assessed immunohistochemis-tically.The change of actin cytoskeleton was shown by fluorescence.Results With the development of hepatic fi-brosis,the positive areas in model groups at week 1 to 4 of ROCK-Ⅰ and α-SMA of the rat livers were larger than that in control group respectively(P <0.05).ROCK-Ⅰ,p-MBS Thr-697 ,α-SMA protein and mRNA were increased than that in control group respectively.ROCK-Ⅰ mRNA expression correlated with α-SMA (r =0.718,P <0.05).With the development of liver fibrosis,the images of fluorescence were inhanced.Conclusion With the develop-ment of liver fibrosis,both protein and mRNA of ROCK-Ⅰ increased.
