Transplantation of marrow mesenchymal stem cells improves the ventricular remodeling and heart functions to acute MI rat models
- VernacularTitle:移植MMSCs改善大鼠实验性心肌梗死后心室重构
- Author:
Xuxian WU
;
Zhixu HE
;
Xiangchun SHEN
;
Zhongjun ZHOU
;
Jian LIU
- Publication Type:Journal Article
- Keywords:
myocardial infarction;
marrow mesenchymal stem cells;
ventricular remodeling
- From:
Basic & Clinical Medicine
2010;30(1):48-53
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of marrow mesenchymal stem cells on heart functions and ventricular remodeling after myocardial infarction in rats. Methods Myocardial infarction model was established by ligation of the left anterior descending coronary artery (LAD) in adult SD rats. 4 and 8 weeks after MMSCs implantation, he-modynamic evaluations, left ventricular weight/body weight ratio and heart weight/body weight ratio were determined. HE staining was performed for counting microvasculars and Van Gieson staining was used for measurements and calculation of the myocardial fibrillar collagen. Then we investigated the migration and evolution of MSCs in vivo by fluorescent microscope. Results HR was significantly decreased in transplantation MMSCs group. Eight weeks after transplantation, body weight in transplantation MMSCs group reached that of control group. At the same time, SBP, DBP and MBP were significantly increased in transplantation MMSCs group. HR was significantly decreased in transplantation MMSCs group. Secondly, left ventricular weight/body weight ratio and heart weight/body weight ratio were significantly decreased 4 weeks after transplantation MMSCs. Then the ratio was significantly decreased 8 weeks after transplantation MMSCs. Thirdly density of microvasculars was increased at the boundary of infarction site in the animals transplanted MMSCs. Finally, total volume of the myocardial fibrillar collagen was reduced in the MMSCs treated groups after MI. Conclusion Transplanting MMSCs can improve the ventricular remodeling and heart functions in acute MI rat models.
