AGEs induces the expression of INOS in cultured cardiac microvascular endothelial cells of rat
- VernacularTitle:AGEs诱发体外大鼠心脏微血管内皮细胞iNOS表达
- Author:
Yongxia CHENG
;
Guibo LIU
;
Sufen GUO
;
Xianghong YANG
- Publication Type:Journal Article
- Keywords:
AGEs;
cardiac microvascular endothelial cells;
NO;
iNOS
- From:
Basic & Clinical Medicine
2010;30(5):515-519
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of the advanced glycation end products(AGEs)on the iNOS expression in cultured cardiac microvascular endothelial cells.Methods Cultured the cardiac microvascular endothelial cells in vitro.After AGEs of the different dose(50～200 mg/L)and different times(0～24 h)played the role on the cells,we determined the NO generation and iNOS protein expression of all groups.Results NO generation and iNOS protein expression increased with the AGEs-dose increasing and treatment time.The results were significantly different.Conclusion These results demonstrate that AGEs may induce iNOS to produce toxic NO in cardiac microvascular endothelial cells,then the diabetic cardiomyopathy occures.
