Aspirin inhibits the proliferation of rat cardiac fibroblasts induced by endothelin-1
- VernacularTitle:阿司匹林抑制内皮素-1诱导的大鼠心脏成纤维细胞增殖
- Author:
Zhiguo ZHANG
;
Li YANG
;
Yaping ZHENG
;
Yanfeng ZHAO
- Publication Type:Journal Article
- Keywords:
endothelin-1;
cardiac fibroblasts;
aspirin;
bradykinin;
nitric oxide
- From:
Basic & Clinical Medicine
2009;29(12):1310-1313
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of aspirin on endothelin-1 induced proliferation of cardiac fibroblasts (CFs). Methods Isolated and cultured CFs from neonatal Sprague-Dawley rats (SD) were randomly divided into 7 groups: control, ET-1, aspirin, ET-1 +aspirin 1 mmol/L, ET-1 +aspirin 2 mmol/L, ET-1 +aspirin 5 mmol/L and ET-1 + aspirinl 10 mmol/L. CFs were counted by MTr assay. Cell cycle distribution was determined with a flow cy-tometer (FCM). [~3H]-Proline incorporation was evaluated by scintillation counting. Nitric oxide (NO) was meas-ured by colorimetry. Results 10~(-7)mol/L ET-1 significantly increased the number of CFs and [~3H]-Pro incorporation and decreased NO_2~-/NO_3~--secretion compared with the control group (P <0.01).1 ～ 10 mmol/L Aspirin inhibited the effects of ET-1 on CFs in a concentration-dependent manner (P <0.05 vs ET-1). In the ET-1 group, the percent-age of cells in the S phase was higher than that of the control, which was inhibited by 10 mmol/L Aspirin (P <0.01 vs ET-1 and control). Conclusion Aspirin can restrain the proliferation and collagen synthesis of cardiac fibroblasts induced by endothelin-1, and this effect may be partially mediated by NO.
