Sequencing analysis on rpoB gene mutation of rifampin-resistance in Mycobacterium tuberculosis
- VernacularTitle:结核分枝杆菌耐利福平rpoB基因突变的序列分析
- Author:
Xin GAN
;
Ling CHEN
;
Jianhua WANG
;
Nana LI
;
Yang CHEN
;
Jianyong ZHANG
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
rifampin;
rpoB gene;
mutation;
drug susceptibility
- From:
Basic & Clinical Medicine
2010;30(3):268-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective Genetic evaluation of relationship between rpoB gene mutation and rifampin-resistance of Mycobacterium tuberculosis (MTB).Methods This study was carded out with 81 clinical isolates and sensitivity test and genotypic analysis by PCR amplification and sequencing of rpoB gene were performed.Results Among these isolates,there were 27 rifampin-resistant and 54 sensitive strains.Of the 27 drug-resistant isolates,20 (74.1%) carried mutations on the amplified fragment of the rpoB gene with 10 mutation types at seven codons including 531 and 526,and two new mutation patterns were recognized.On the uther hand,one mutation (1.9%) appeared in 54 drug-sensitive strains.Conclusion The study shows geographical variation in the mutation types of rpoB gene in M.tuberculosis isolates from Guizhou,China.The result is valuable for the development of fast diagnostic methods.
