Effects of aspirin on [Ca~(2+)] i and NGB in cultured neural cells of newborn rat under chemical-induced hypoxic exposure
- VernacularTitle:阿司匹林对化学性缺氧状态下新生大鼠脑神经细胞[Ca~(2+)]i和NGB的影响
- Author:
Ju CHENG
;
Decheng BAI
;
Kun XIE
;
Min HOU
- Publication Type:Journal Article
- Keywords:
aspirin;
primary cell culture;
hypoxia;
intracellular free calcium concentration;
neuroglobin
- From:
Basic & Clinical Medicine
2009;29(11):1180-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of aspirin on hypoxic brain neural cells of rat and the effects of extracellular calcium on the neuroprotective effect of aspirin. Methods The hypoxic cell model was established by adding Na_2S_2O_4 to the calcium or calcium free medium. Cultured primary cortical neurons of rat were pretreated with ASA in vitro. The change of intracellular free calcium and neuroglobin (NGB) were observed and analyzed by laser scanning confocal microscope. Results The expression level of [Ca~(2+)] i and NGB increased significantly in the chemical hypoxia group ( P < 0. 05 ). ASA can attenuate the increase of [ Ca~(2+) ] i and NGB in the hypoxic group and calcium-free hypoxia group(P <0. 05). Conclusion Aspirin can inhibit calcium overload and the hypoxia-induced expression of NGB, so protect rat brain cells against hypoxia.
