Neural cell induction with human placenta derived adherent cells
- VernacularTitle:人胎盘贴壁细胞定向诱导神经样细胞
- Author:
Yuesi WANG
;
Fangang MENG
;
Yanwei WANG
;
Jianyuan LI
- Publication Type:Journal Article
- Keywords:
placenta adherent cells;
neural cell;
mesenchymal stem cell;
differentiation
- From:
Basic & Clinical Medicine
2009;29(12):1258-1262
- CountryChina
- Language:Chinese
-
Abstract:
Objective The expression of hematopoietic stem cell and neural markers on placenta adherent cells and their association were investigated, the potential of neural differentiation of placenta adherent cells may demonstrate the relationship between placenta adherent cells and hematogenesis and neurogenesis. Methods Isolated adherent cells in human placenta tissue were stained immunocytochemically with Nestin, MAP_2, MBP, CD133, CD34 antibody to detect protein production and induced neural differentiation with β-mercaptoethanol and retinoic acid. Results Isolated placenta adherent cells confirmed to be heterogenous expressed CD133, Nestin, MAP_2 weakly and CD34 none. While induced cells showed neural like morphology, strong positive for Nestin, MAP_2 and weak positive for MBP and GFAP in immunocytochemical staining. Conclusion Human placenta adherent ceils can be induced into neural like cells in vitro with histological characteristics of mesenchymal stem cell(MSC) ,it provides a new source for clinical application of hMSCs.
