Enhancement of Y14 and Upf1 expression in human breast cancer cell lines
- VernacularTitle:Y14和Upf1在人乳腺癌细胞表达增强
- Author:
Liang RAN
;
Gang TU
;
Xiaoyi WANG
;
Cong ZHAO
;
Guosheng REN
- Publication Type:Journal Article
- Keywords:
NMD;
Y14;
Upf1;
human breast cancer cell line;
human breast cancer tissue
- From:
Basic & Clinical Medicine
2009;29(11):1150-1154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study expression enhancement and significance of Y14 and Upf1 in human breast cancer cell lines and tissue. Methods Immuocytochemistry and laser scanning confocal microscope(LSCM) were applied. Y14 and Upf1 were determined in human breast cancer cell lines(MCF-7,ZR-75-30,T47D,MDA-MB435s,MDA-MB-453, MDA-MB-231) and breast epithelial cell line ( HBL-100). Results (1) Y14 and Upf1 level of breast cancer cells are obviously higher than that in breast epithelial cell line (P < 0. 05 ). (2)Y14 and Upf1 level of MDA-MB-231 are obviously higher than that in MCF-7. (3)The expression enhancement of Y14 and Upf1 level are obviously higher in human breast cancer tissue. Conclusion The expression level of Y14 and Upf1 in breast cancer cells and tissue enhance obviously.
