Complement mediated killing of human glomerular mesangial cell by Fcα/μ receptor
- VernacularTitle:补体介导Fcα/μ受体对人肾小球系膜细胞的杀伤
- Author:
Lian SHEN
;
Xiaoyan WANG
;
Qing ZHAO
;
Wei ZHANG
- Publication Type:Journal Article
- Keywords:
Fcα/μ receptor;
IgM;
immune complexes;
complement;
glomerular mesangial cell
- From:
Basic & Clinical Medicine
2010;30(5):471-475
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether Fcα/μ receptor(Fcα/μR)can mediate complement killing of human glomerular mesangial cells.Methods Fcα/μR cDNA contained plasmid,pcDNA3.1-Fcα/μR was transfected into a human glomerular mesangial cell(NHMC).Fcα/μR expression was detected by Western blot and laser scanning eonfocal microscopy.Binding of IgM-immune complexes(IgM-IC)to the Fcα/μR on cell membrane was detected by flowcytometry and laser scanning confocal microscopy.Killing of cells by complement was shown by Trypan blue exclusion assay.Results NHMC cells transfected with Fcα/μR could bind IgM and IgM-IC.After treatment with complement,added IgM-IC,the death rate of pcDNA3.1-Fcα/μR transfected cell was significant higher than the control groups of wild type cell,pcDNA3.1 transfected cell and the pcDNA3.1-Fcα/μR transfected cell without IgM-IC.Conclusion IgM-IC can bind to the Fcα/μR expressed NHMC cells and mediate complement killing of the cells.
