Relationship between human membrane associated sialidase ( Neu3 ) and multidrug resistance (MDR) in K562 cell line
- VernacularTitle:人质膜型唾液酸酶与人红白血病细胞多药耐药相关
- Author:
Li XU
;
Liankun SUN
;
Feng LI
;
Bin YU
;
Xiaojie LI
;
Xuejian ZHAO
;
Yang LI
- Publication Type:Journal Article
- Keywords:
human membrane associated sialidase;
muhidrug resistance;
K562
- From:
Basic & Clinical Medicine
2010;30(2):139-143
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of human membrane associated sialidase (Neu3) in multidrug resistance ( MDR) of K562 cells. Methods K562 cells and K562/ADM cells were treated with DNR alone or with combination of DNR and NeuAC2en. The cell survival rate was measured by MTT assay; the expression of MDR related factors and apoptosis related factors were determined by Western blot and RT-PCR; Neu3 activity was detected by TBA reaction. Results The survival rate of K562/ADM cells was higher than that of K562 cells; NeuAC2en showed synergistic effect with DNR(P <0. 01) ; Neu3 activity of K562 and K562/ADM cells decreased after DNR or NeuAC2en induction and the decrease was most significant in the combination treatment group (P <0. 01). Under the identical condition, the protein expression of P-gp and Neu3 and the mRNA expression of MDR1, Neu3, BCL-xl and BCL-2 increased comparing with K562 cells. The mRNA level of BAX in K562 and K562/ADM groupsdid not changed significantly. MDR1, Neu3,BCL-xl and BCL-2 decreased after DNR induction and down-regulated most obviously in the groups treated by DNR combined with NeuAC2en. Conclusion Neu3 may be related with multidrug resistance of K562/ADM cell through regulating apoptosis and the expression of MDR1.
