VEGF-induces up-expression of Survivin in differernt types of cell
- VernacularTitle:VEGF上调不同类型细胞Survivin的表达
- Author:
Xuehu WANG
;
Zhongxue FU
;
Wei SHEN
;
Xingye WU
- Publication Type:Journal Article
- Keywords:
vascular endothelial growth factor;
Survivin;
colon cancer;
induce
- From:
Basic & Clinical Medicine
2009;29(12):1239-1243
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of VEGF-induced expression of Survivin by colon cancer cells (LOVO, HCT116) and human umbilical vein endothelial cells (HUVECs). Methods VEGF was used to stimu-late the LOVO, HCT116, HUVECs. We used RT-PCR and Western blot to estimate the expression of Survivin in different cells, and immunocytochemistry to investigate the expression level and site of Survivin protein. Results Survivin was expressed highly in the cytoplasm and was upregulated with the induction of VEGF. In HUVECs, Sur-vivin expression was not obvious but significantly upregulated with the inductioin of VEGF in the cytoplasm and nu-cleus. Conclusion The expression of Survivin mRNA and protein are upregulated by VEGF stimulation. The level and distribution of Survivin are different in tumor cell lines and non-tumorgenicity cells.
