Hepatocyte growth factor attenuates hypoxia/reoxygenation injury in cortical neurons
- VernacularTitle:肝细胞生长因子减轻皮质神经元的缺氧/复氧损伤
- Author:
Zhixing HU
;
Jumin GENG
;
Daoming LIANG
;
Lanou WU
;
Chunlan ZHENG
;
Haiyun LUO
;
Jian TAO
- Publication Type:Journal Article
- Keywords:
hepatocyte growth factor;
hypozia/reoxygenation;
apoptosis;
PI3K/Akt;
caspase-3
- From:
Basic & Clinical Medicine
2010;30(4):369-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hepatocyte growth factor (HGF) on cultured Sprague-Dawley rat cortical neurons injured through hypoxia/reoxygenation.Methods Primary cultured cerebral cortical neurons were isolated from newborn rots.Neurons were pre-incubated with different concentrations (15,30 and 60 μg/L) of HGF.The cell viability was detected by MTT.Apoptosis was measured by Hoechst 33258 staining and flow cytometer.Lactate dehydrogenase (LDH) and caspase-3 activity were determined by colorimetry.Results Compared with normal group,hypoxia/reoxygenation treatment significantly decreased cell viability,increased LDH activity and the percentage of apoptotic cells.Pretreatment of HGF for 12 h could remarkably reverse the decrease of cell viability and the increase of apoptosis rate in neurons induced by hypoxia/reoxygenation treatment.HGF pre-treatment also attenuated the activity of LDH and caspase-3 in a dose-dependent manner.The effects of HGF could be inhibited by a special PI3K/Akt pathway inhibitor,LY294002.Condusion HGF could attenuate rat cortical neuron injury induced by hypoxia/reoxygenation.The neuroprotective effect of HGF may be related to activating PI3K/Akt pathway,and further suppressing the expression of caspase-3.
