Effect of chronic intermittent hypoxia on the expression of fractalkine in rat liver
10.3969/j.issn.1672-7347.2013.10.002
- VernacularTitle:慢性间歇低氧对大鼠肝fractalkine表达的影响
- Author:
Yayong LI
;
Yina WANG
;
Yu YANG
;
Yingquan LUO
;
Ping CHEN
- Publication Type:Journal Article
- Keywords:
obstructive sleep apnea-hypopnea syndrome;
chronic intermittent hypoxia;
liver injury;
fractalkine
- From:
Journal of Central South University(Medical Sciences)
2013;38(10):984-990
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of chronic intermittent hypoxia (CIH) on liver injury and the expression of fractalkine in rats and explore its possible mechanism.
Methods: A CIH murine model was established to mimic the pathophysiology of obstructive sleep apnea-hypopnea syndrome (OSAHS) in humans. Thirty healthy male Spraque-Dawley rats were randomly assigned to 3 groups: a 5% CIH group, a 5% CIH+RH (removal of hypoxia) group and a control group ( 10 rats in each group). The 5% CIH and 5% CIH+RH groups were exposed to CIH for 3 weeks, 8 h/d, and the frequency of hypoxia was 20 times/h. The 5% CIH+RH group was then exposed to normal gaseous environment for another 3 weeks. After the experiment, liver sections were stained with hematoxylin-eosin (HE) and the liver pathology was observed. The expression of fractalkine in the liver tissues was detected by immunohistochemical method.
Results: 1) Compared with the control group, the hepatic steatosis and inflammatory activities in the 5% CIH and 5% CIH+RH groups were more severe (allP<0.01 ); compared with the 5% CIH group, the hepatic steatosis and inflammatory activity in the 5% CIH+RH group were dramatically reduced (P<0.01 ). 2) Compared with the control group, the fractalkine expression in the 5%CIH and 5% CIH+RH groups was increased (bothP<0.01). The fractalkine expression in the 5% CIH+RH group was dramatically downregulated compared with that in the 5% CIH group (P<0.01).
Conclusion: CIH can induce liver injury and high fractalkine expression in rat liver tissues.
