Change of serum levels of matrix metalloproteinase-13 and its inhibitor factor during bone metabolism in Chinese postmenopausal women
10.3760/cma.j.issn.1008-6315.2013.10.002
- VernacularTitle:血清基质金属蛋白酶13及其抑制因子在绝经后妇女骨代谢中的浓度变化
- Author:
Yi DAI
;
Ning GAN
- Publication Type:Journal Article
- Keywords:
Matrix;
metalloproteinase;
13;
Tissue;
inhibitor;
of;
matrix;
metalloproteinase;
1;
Bone;
metabolism;
Osteoporosis;
Postmenopausal
- From:
Clinical Medicine of China
2013;29(10):1013-1016
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of the serum matrix metalloproteinase-13 (MMP-13) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and the correlations of MMP-13 and TIMP-1 with bone metabolic markers or bone mineral density(BMD) in aged postmenopausal Chinese women.Methods One hundred and twenty women aged 48-65 years with postmenopausal were selected as subjects.They were divided into normal group(n =28),low bone mass group (n =36) and osteoporosis group (n =56) accordance with the WHO standard.The levels of serum MMP-13,TIMP-1,estradiol (E2),Osteoprotegerin (OPG),Osteoprotegerin ligand (OPGL),Procollagen terminal propeptide of N (PINP),and Type Ⅰ collagen cross-linked C terminal peptide(CTX) were measured using enzyme linked immunosorbent assay(ELISA).And the ratios of MMP-13 to TIMP-1 were calculated.Bone mineral density(BMD) of the lumbar spine,femoral neck,Fahrenheit and greater trochanter were measured using dual energy X-ray absorptiometry.Results There was significant difference of serum MMP-13 concentrations in normal controls,low bone mass group and osteoporosis group((27.08 ± 1.41) μg/L,(45.64 ± 1.62) μg/L and (44.25 ± 1.21) μg/L; F =110.314,P =000),and serum MMP-13 concentrations in low bone mass group and osteoporosis group higher than that in normal controls (P ＜ 0.05),serum MMP-13 concentrations in low bone mass group higher than that in osteoporosis group,but there was on significant difference(P ＞ 0.05).But Serum TIMP-1 concentrations in three groups was no significant difference (F =10.721,P =0.801).The ratio between MMP-13 and IIMP-1 was 0.185 ± 0.062,0.311 ± 0.053,0.332 ± 0.063 respectively,and there was significant difference of the three groups (F =137.771,P =0.000),and the ratio of the low bone mass group and osteoporosis group higher than the normal group(P ＜0.05),but there was no significant difference between the two groups.In osteoporosis group,Negative correlations between MMP-13,and BMD,E2 and OPGL,were found (r =-0.296,-0.198,-0.301,-0.298,-0.233 respectively,P ＜ 0.05).Meanwhile positive relations were found between MMP-13 and OPG,PINP,CTX (r =0.228,0.315,0.312 respectively,P ＜ 0.05).In low bone mass group,the level of MMP-13 was significantly relative with BMD (the lumbar spine,Fahrenheit),E2 and CTX (r =-0.188,-0.196,-0.235,0.289 respectively,P ＜ 0.05).Conclusion There are significant correlations between serum MMP-13,ratios of MMP-13 and TIMP-1 and bone metabolism marker and BMD.MMP-13 level may increase with increases in osteoporosis and osteopenia.The increases of serum MMP-13 and the decrease of ratio of MMP-13 to TIMP-1 might be biomarkers in high bone turnover state,such as postmenopausal osteoporosis and early stage of osteopenia.
