A method for elevating survival rate of models of steroid-induced necrosis of femoral head
10.3969/j.issn.2095-4344.2013.50.017
- VernacularTitle:提高激素性股骨头坏死模型成活率的方法
- Author:
Ruiqi LI
;
Guoping ZHANG
;
Yijiong LI
;
Yali LI
;
Lizhong REN
;
Yuchen ZHANG
;
Wei WANG
;
Hongyang GAO
;
Yajun Lü
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(50):8729-8734
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Animal death is a main influential factor for experimental results in establishment of animal models of steroid-induced necrosis of femoral head.
OBJECTIVE:To observe models of femoral head necrosis established using lipopolysaccharide and dexamethasone so as to elevate success rate of model induction.
METHODS:A total of 48 New Zealand white rabbits were equal y and randomly divided into model group, gentamicin group, gentamicin+lansoprazole group and control group. The first three groups were injected with lipopolysaccharide for 2 consecutive days via the ear vein, and then they were injected with dexamethasone via intramuscular injection in the buttocks for 3 consecutive days to establish models of femoral head necrosis. The rabbits of gentamicin group, gentamicin+lansoprazole group were intragastrical y administered gentamicin for 7 consecutive days after success model induction. Simultaneously, gentamicin+lansoprazole group received intramuscular injection with lansoprazole. Rabbits in the control group were only injected with saline.
RESULTS AND CONCLUSION:Models were successful y established in the model, gentamicin, gentamicin+lansoprazole groups. Their conditions were best in the gentamicin+lansoprazole group. Mortalities in above-mentioned groups were 33.3%, 25%and 8.3%, respectively. Significant differences in the number of dead rabbits were detected in the model, gentamicin, gentamicin+lansoprazole and control groups (P<0.05). Results indicated that the combined use of gentamicin and lansoprazole can elevate survival rate of experimental animals during the establishment of rabbit models of steroid-induced necrosis of femoral head.
