Some leukocyte differentiation antigens express in side population cells in leukemia K562 cell line
10.3969/j.issn.2095-4344.2013.49.021
- VernacularTitle:K562细胞株侧群细胞中部分白细胞分化抗原的表达
- Author:
Ruihua FAN
;
Dianxuan GUO
;
Tiecheng ZHANG
;
Shun YUE
;
Mingde HUANG
;
Rong YAO
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(49):8596-8601
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:To study the phenotypes of side population cells in leukemia is important for understanding the heterogeneity and origin of tumor cells, molecular markers and targeted therapy.
OBJECTIVE:To identify whether the human chronic myeloid leukemia cellline-K562 contains side population cells or not, and to further observe the differences in expressions of leukocyte differentiation antigens from side population cellsubset and non-side population cells subset.
METHODS:Flow cytometry was used to detect whether there were side population cells in the K562 celllines. Then, the expression of CD34+, CD34+CD38-, CD34+CD38+, HLA-DR+cells in the side population subsets and non-side population subsets.
RESULTS AND CONCLUSION:Flow cytometry results showed that the K562 cellline contained side population cells, and the proportion of side population cells was much lower. The side population cells accounted for (2.7±0.5)%of viable cells in K562. The expressions of CD34+cells and CD34+CD38-cells in the side population subset were significantly higher than those in the non-side population subsets. The expressions of CD34+CD38+cells and HLA-DR+cells in the side population subset and non-side population subset did not have a significant difference. Heterogeneity was found in the differentiation antigen expression between the side population subset and non-side population subset.
