Compatibility of human osteoblasts and composite porous bioscaffold
10.3969/j.issn.2095-4344.2013.51.014
- VernacularTitle:人成骨细胞与复合型多孔生物支架降解产物的相容性
- Author:
Jiangwei SUN
;
Dong WANG
;
Haiyu SUN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(51):8869-8874
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:The degradation of a col agen Ⅰ modified porous calcium sulfate scaffold in vivo is unclear, and its degradation product effects on human osteoblasts are rarely reported.
OBJECTIVE:To observe the biocompatibility of human osteoblasts with degradation products of calcium sulfate/col agen membrane composite porous scaffold.
METHODS:Passage 2 human osteoblasts were cultured in the extract of degradation products of calcium sulfate/col agen membrane composite porous scaffold and in Dulbecco’s modified Eagle’s medium containing 10%newborn calf serum. At days 1, 3, 5, 7, cellproliferative curves and total protein were determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method and Coomassie bril iant blue micro-plate method, respectively. And alkaline phosphatase activity was also detected.
RESULTS AND CONCLUSION:The proliferation rate of human osteoblasts in the extract of degradation products of calcium sulfate/col agen membrane composite porous scaffold was slightly higher than that in the Dulbecco’s modified Eagle’s medium containing 10%newborn calf serum, but there was no significant difference (P>0.05). Alkaline phosphatase activity, total protein synthesis and alkaline phosphatase/total protein were increased with time in the two groups, but there was no significant difference at different time (P>0.05). These findings indicate that the degradation products of calcium sulfate/col agen membrane composite porous scaffold cannot influence proliferation and growth of human osteoblasts as wel as their normal physiological functions, which have good biocompatibility.
