Primary application of WCX magnetic beads combined with MALDI-TOF MS in detecting differentially expressed proteins in renal clear cell carcinoma patient's urine
10.3760/cma.j.issn.1000-6702.2013.12.006
- VernacularTitle:磁珠联合基质辅助激光解吸附电离飞行时间质谱技术对肾透明细胞癌尿液差异蛋白的初步研究
- Author:
Dexin DONG
;
Hanzhong LI
;
Weigang YAN
;
Zhigang JI
;
Yushi ZHANG
;
Weifeng XU
- Publication Type:Journal Article
- Keywords:
Renal clear cell carcinoma;
Proteomics;
Magnetic beads;
Urine
- From:
Chinese Journal of Urology
2013;34(12):912-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the application of proteomics in detecting differentially expressed proteins in renal clear cell carcinoma (RCCC) patient's urine in order to improve the diagnosis rate of RCCC.Methods From Mar.2010 to May.2010,the urine samples of 11 RCCC cases were collected,including 10 males and 1 female with average age of 63 (46-78) years.All patients were finally diagnosed as RCCC by post-operative or biopsy pathology.The normal control urine samples were collected from 10 males with average age of 29 (25-32) years.WCX beads combined with matrix assisted laser desorption ionization time of flighl mass spectrometry (MALDI-TOF MS) technique was applied in detecting differentially expressed proteins in RCCC patient's urine to find out differentially expressed proteins.And genetic algorithm was utilized to establish a diagnosis model.Results 160 differentially expressed proteins in RCCC patient 's urine were detected,and 1 was in significant difference,P=0.0304.ClinProTools 2.2 software was utilized with genetic algorithm to find out 13 differentially expressed proteins to establish a diagnosis model,and the sensitivity and specificity rate was 100％ after cross validation.Conclusions The diagnosis model established by genetic algorithms has high sensitivity and specificity rate,and can improve the diagnosis of RCCC.
