Features of the NCAM+c-Kit+ subset of hepatic progenitor cells in intrahepatic cholangiocarcinoma
10.3760/cma.j.issn.1007-8118.2013.12.015
- VernacularTitle:NCAM+c-Kit+胆管癌细胞的肝脏祖细胞特性研究
- Author:
Yanling HE
;
Jing XU
;
Lihong YANG
;
Lingmin LI
;
Yanhong TAN
;
Xiuhua CHEN
;
Zhifang XU
- Publication Type:Journal Article
- Keywords:
Bile duct neoplasms;
Neural cell adhesion molecules;
Neoplasm transplantation
- From:
Chinese Journal of Hepatobiliary Surgery
2013;19(12):934-937
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the features of the NCAM+ c-Kit+ subset of hepatic progenitor cells in the intrahepatic cholangiocarcinoma (ICC) cell line RBE.Method Magnetic activated cell sorting was used to isolate NCAM+ c-Kit+/NCAM-c-Kit-subset cells,which were tested for hepatic progenitor cell properties and proliferation,colony formation,and invasive abilities in nude mice.Resuits The cell proliferation ability of NCAM+c-Kit+ subset cells was stronger than that of NCAMc-Kit-subset cells (P＜0.01).In serum-free condition,the number of colonies formed by NCAM+c-Kit+ subset cells was more than that of NCAM-c-Kit-cells (P＜0.01).1 × 104 NCAM+c-Kit+ cells were enough to form tumors in nude mice after subcutaneous inoculation for two weeks,while 1 × 106 NCAM-c-Kit-cells were necessary to form tumors for three weeks.The tumor formation rate of NCAM+c-Kit+ cells was higher than that of NCAM-c-Kit-cells (P=0.04).Conclusions It is possible that NCAM+c-Kit+ subset cells in RBE have the properties of hepatic progenitor cells,and NCAM combined with c-Kit might be a valuable marker for isolating and purifying ICC stem/progenitor cells.
