Analysis of epidermal growth factor receptor expression and gene expression status in tissue microarray of cervical squamous cell carcinoma
10.3760/cma.j.issn.0529-567x.2013.11.010
- VernacularTitle:子宫颈鳞癌组织中表皮生长因子受体的表达及其基因状态分析
- Author:
Suying ZHOU
;
Guofei FENG
;
Guorong CHEN
;
Dan PAN
;
Pinnan ZHANG
;
Xiaomin YANG
;
Zuoli XIA
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
Carcinoma,squamous cell;
Receptor,epidermal growth factor;
Gene amplification;
In situ hybridization,fluorescence;
Immunohistochemistry
- From:
Chinese Journal of Obstetrics and Gynecology
2013;48(11):843-846
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the protein expression and gene expression status and the relationship between epidermal growth factor receptor (EGFR) protein expression and EGFR gene status.Methods Tissue microarray containing 72 cervical squamous cell carcinoma tissues was constructed,and EGFR protein expression and gene status were evaluated by immunohistochemical and fluorescence in situ hybridization (FISH) techniques.Results Protein expression of EGFR:69 of 72 cervical squamous cell carcinomas were observed.The results demonstrated it was significant association with invasion depth,lymphnode metastasis and lymph-vessel invasion (x2 =4.998,P ＜ 0.05 ; x2 =4.299,P ＜ 0.05 ; x2 =4.686,P ＜ 0.05) in cervical squamous cell carcinomas.For FISH assessing EGFR gene,64 of 72 carcinomas were observed; 7 of 64 cases showed EGFR gene amplification,and 25 disomy,23 trisomy and 9 polysomy were detected.There were high levels of protein expression in all the EGFR gene amplification cases,and there were significant association between EGFR protein expression and the gene copy number (x2 =13.564,P ＜ 0.05).Conclusions EGFR may participate in the occurrence,progression and metastasis of cervical squamous cell carcinoma.Overexpression of EGFR protein may result from gene amplification and gene copy number increases,which showed that EGFR gene expression status may be a more effective biological indicator of cervical squamous cell carcinoma targeted therapy.
