Detection of the specific antigens and viral RNA in the tissues of the BALB/c mice infected with hantavirus

VernacularTitle:汉坦病毒感染BALB/c小鼠组织中特异性抗原及病毒RNA的检测
Author: Yongni XU ; Zhicha HU ; Linfeng CHENG ; Fanglin ZHANG
Publication Type:Journal Article
Keywords: Hantavirus infections; Mice,inbred BALB C; Antigens,viral; RNA,viral
From: Journal of Chinese Physician 2013;15(11):1441-1445
CountryChina
Language:Chinese
Abstract: Objective To establish an evaluation system about animals infected with hantavirus,an observation of the BALB/c mice infected with hantavirus was made.Methods BALB/c mice were infected with hantavirus by intramuscular injection with stock solution.The specific antigen from BALB/c mice tissues after 3 days was detected with enzyme-linked immunosorbent assay (ELISA) and viral RNA with real-time polymerase chain reaction (RT-PCR).Results Within a short term,the specific antigen and viral RNA were detected from the brain and liver at day 3 after infection,but not be detected from the heart,spleen,lung,and kidney samples.Conclusions The results provided ones with some information on animals infected with hantavirus.