Correlation between peripheral blood inflammatory cytokines and kainic acid-induced seizure severity in rats
10.3760/cma.j.issn.1674-6554.2013.11.003
- VernacularTitle:红藻氨酸致痫大鼠外周血炎性因子与癫痫发作程度的相关性研究
- Author:
Xiaoming FENG
;
Jianxia MA
;
Xiaohong LUO
;
Jianjun LUO
;
Lu LU
;
Tingxian NIU
- Publication Type:Journal Article
- Keywords:
Kainic acid;
Epilepsy;
Seizure severity;
Inflammatory cytokines
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;22(11):969-971
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To investigate the correlation between the level of peripheral blood inflammatory cytokines and kainic acid-induced seizure severity in rats.Methods 140 rats were divided into control and model group randomly,70 rats in each group.Model group rats were injected with kainic acid (10 mg/kg) by intraperitoneal,and the control rats were injected with sodium chloride.The change of their behaviors was observed and the concentrations of TNF-α,IL-1β,IL-4 and IL-10 were determined by ELISA in each group at different times.Results The rats showed epilepsy grand mal in 3 h-9 h after KA injection.The concentrations of TNF-α and IL-1β in model groups were significantly higher than those in control group in 6 h-12 h (6 h:(21.5±3.2) pg/ml vs (12.3±3.1)pg/ml;12 h:(20.6±4.2)pg/ml vs (11.5±3.8)pg/ml)(P＜0.05) and IL-4 in model group was higher at only 12 h ((53.55±3.08) pg/ml vs (33.26±4.16)pg/ml) (P＜0.05).The level of IL-10 in model groups was not statistically significant compared with control group (P＞0.05).Conclusion The proinflammatory cytokines (TNF-α and IL-l β) participate in the seizure procedure,meanwhile their levels and seizure severity have eminent correlations,but antiinflammatory cytokines (IL-4 and IL-10) had not.
