Effects of KAI1 gene transfection on proliferation, migration, invasion and VEGF expression of pancreatic cancer MiaPaCa-2 cells under hypoxic condition
10.3760/cma.j.issn.1674-1935.2013.06.003
- VernacularTitle:KAI1基因转染对乏氧培养下胰腺癌MiaPaCa-2细胞增殖、迁移及VEGF表达的影响
- Author:
Xu LIU
;
Xiaozhong GUO
;
Hongyu LI
;
Jiang CHEN
;
Wenda XU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Anoxia;
Cell proliferation;
Cell movement;
Vascular endothelial growth factors;
KAI1 gene
- From:
Chinese Journal of Pancreatology
2013;13(6):370-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of KAll gene transfection on proliferation,migration,invasion and VEGF expression of pancreatic cancer MiaPaCa-2 cells under hypoxic condition,and explore possible mechanism.Methods The pcMV-KAI1 vector which contained the full length of KAI1 cDNA was transfected into pancreatic cancer cells MiaPaCa-2,and KAI1,VEGF C,VEGF A protein expressions were determined by Western blot.The proliferation of pancreatic cancer cells was evaluated by MTT method.Wound-healing assay and cell invasion assay were used to detect the migration and invasion of pancreatic cancer cells.The expression of VEGF C,VEGF A in supernatant of culture was measured by ELISA method.Results The expression of KAI1 protein in MiaPaCa-2 K transfected with KAI1 was significantly higher than that in nontransfected cells [(0.549 ± 0.021) vs 0,P＜0.05].The proliferation under hypoxic condition was not significantly different,but the migration distance was significantly shorter and the number of transmembrane cell was significantly decreased [(14.0 ± 5.8) vs (43.0 ± 14.4),P ＜ 0.05].The expression of VEGF-C in cell was significantly decreased [(0.218 ± 0.043) vs (0.745 ± 0.069),P ＜0.05],but the expression of VEGF-A was not significantly different; the expression of VEGF-C in cell culture supernatants was significantly decreased [(1236 ± 247) vs (2045 ± 221) pg/ml,P ＜ 0.01].Conclusions The migration,invasion ability of pancreatic cancer MiaPaCa-2 cells with KAll transfection under hypoxic condition is decreased,and the possible mechanism of inhibiting lymphatic metastasis is down-regulation of VEGF-C expression.
