Effects of miR-10a down-regulated by siRNA on migration and invasion of human pancreatic cancer cell AsPC-1
10.3760/cma.j.issn.1674-1935.2013.06.004
- VernacularTitle:下调miR-10a表达对人胰腺癌细胞AsPC-1迁移和侵袭的影响
- Author:
Heng ZHANG
;
Huiyong PENG
;
Changfeng MAN
;
Juan XU
;
Weidong QI
;
Pengcheng JIANG
;
Yu FAN
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
MicroRNAs;
Cell movement;
Neoplasm invasiveness;
Matrix metalloproteinase-13;
miR-10a
- From:
Chinese Journal of Pancreatology
2013;13(6):374-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-10a expression on migration and invasion of human pancreatic cancer cells AsPC-1.Methods Small interfering RNA targeting at miR-10a (miR-10a-siRNA) was constructed,then it was transfected into pancreatic cancer AsPC-1 cells,and nonsense siRNA (Nc-siRNA) group and blank control group was established.Real time PCR assay was used to detect the expression of miR-10a in the 3 groups,and wound healing assay and Transwell assay were used to determine the migration and invasion abilities of cancer cells.The amount of matrix metalloproteinase-13 (MMP-13) in supernatant of cancer cell culture of each group was examined by ELISA assay.Results The miR-10a levels in control group,NC-siRNA group and miR-10a-siRNA group were 1.05 ±0.08,1.03 ±0.06,0.02 ±0.01 ; and the number of transmembrane cell were (150 ± 2.6),(145 ± 2.2),(62 ± 1.8),the levels of MMP-13 in the supernatant were (108.5 ± 2.8),(107.8 ± 2.5),(35.8 ± 1.5) pg/ml.The values were significantly lower in miR-10a-siRNA group than those in control group and NC-siRNA group (P ＜ 0.01).The distance of cultured clone in miR-10a treated cancer cells (736± 18 μm) was significantly longer than those in the controls (385 ±5 μm) and NC-siRNA group (395± 13 μm,P＜0.01).Conclusions Down-regulation of miR-10a by siRNA may inhibit migration and invasion of pancreatic cancer AsPC-1 cells,and the downregulated expression of MMP-13 may be one of the important mechanisms.
