Experimental Study of Optimized Compatibility Component of Water Extractives of Liqi Huoxue Medicinals by Uniform Designed Method
10.11842/wst.2013.09.004
- VernacularTitle:均匀设计筛选理气活血中药提取物优化配伍的实验研究
- Author:
Jiangang LIU
;
Lubo MA
;
Dawu ZHANG
;
Dazhuo SHI
;
Yi WANG
;
Yiyu CHENG
- Publication Type:Journal Article
- Keywords:
Qixue Bingzhi Fang;
Xuefu Zhuyu Tang;
hyperlipidemia;
platelet function;
inflammatory factor;
vasoactive substances;
uniform design method
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1882-1889
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was designed to search and identify the active principle as well as the optimal proportion of water-soluble extractives of traditional Chinese medicine (water-soluble extractives) Liqi Huoxue medicinals com-patibility (Qixue Bingzhi Fang-CWQB) in the prevention and treatment of atherosclerosis (As) by optimal uniform design method. The water-soluble extractives of CWQB were divided into 6 sections (A, B, C, D, E, F) through macroporous resin. The effect intensity and step of every component were compared through its effect on blood fat level, platelet aggregation, inflammatory factors, vascular endothelial growth factor (VEGF) and so on among hyper-lipoidemia rat models. The pharmacological experimental results and statistical analysis showed that CWQB water-soluble extractives of component D (mainly is paeoniflorin, accounted for 49.12%), component E (mainly is total flavonoids, accounted for 30.0%) compatibility had better effects on decreasing blood fat and triglyceride (TG). Com-pared with the model group, there was significant difference (P < 0.01). It also had inhibiting effect on endothelin (ET) and maximum platelet aggregation rate (P < 0.01). The component F (mainly is total acids, accounted for 32.7%) had inhibiting effect on serum IL-6 and IL-8 (P< 0.01). It was concluded that different compatibility of wa-ter-soluble extractives of CWQB can be applied to different targets or steps of the body. The active principle extrac-tives include main component of paeoniflorin, flavonoids and total acids. The best proportion is about 1:1:1.
