ISSR Analysis on Genetic Diversity of Germplasm Resource of Lonicerae Japonicae Flos
10.11842/wst.2013.09.005
- VernacularTitle:金银花种质资源遗传多样性的ISSR分析
- Author:
Zhiying SUN
;
Hui YAO
;
Zhenzhong WANG
;
Yuan BI
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Lonicerae Japonicae Flos;
ISSR;
germplasm;
genetic diversity
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1890-1895
- CountryChina
- Language:Chinese
-
Abstract:
This paper was aimed to study the genetic diversity and genetic relationship of germplasm resource of Lonicerae Japonicae Flos in order to provide references for its breeding. A total of 36 samples from 18 farm varieties and wild species, as well as related species of Lonicera japonica Thunb. from the main production areas were studied by ISSR-PCR markers. The Jaccard coefficient was worked out by NTSYS-pc software. And a cluster dendrogram of different samples was established based on unweighted pair-group method with arithmetic mean (UPGMA). The re-sults showed that 12 ISSR primers generated 129 loci of which 114 loci were polymorphic. The average percentage of polymorphic bands (PPB) is 88.37%. In the cluster dendrogram, different samples of Lonicera japonica are in the same group, which showed that it is a natural species; the wild sample is separated from the cultivated samples; the traditional type-Maohua is relative stable, but the type of Jizhuahua includes complicated varieties, and it has obvi-ous genetic variation; the new variety Jiufeng 1 has already distinct into one stable type. It was concluded that the ISSR method was suitable for germplasm identification, genetic diversity analysis of Lonicerae Japonicae Flos, thus providing a theoretical foundation for its cultivation and breeding.
