Identification of Original Plant of Plantaginis Semen and Its Adulterants Using DNA Barcoding Method Based on ITS2 Sequence
10.11842/wst.2013.09.006
- VernacularTitle:基于ITS2序列的车前子原植物及其混伪品的分子鉴定
- Author:
Lan WU
;
Yimei LIU
;
Yongxing XIONG
;
Keli CHEN
- Publication Type:Journal Article
- Keywords:
Plantaginis Semen;
ITS2 sequence;
adulterants;
identification
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1896-1900
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore a new method to identify the original plant of Plantaginis Semen and its adulterants by the ITS2 regions. The second internal transcribed spacer (ITS2) of ribosomal DNA was amplified and sequenced by bidirectional sequencing of PCR products. Sequence assembly and consensus sequence generation were performed by using CodonCode Aligner. The ITS2 secondary structure was predicted using ITS2 database and web-sites. The phylogenetic tree was constructed by MEGA5. The results showed that the maximum intraspecific K2P dis-tance of Plantago asiatica was 0.009 9, while the minimum interspecific K2P distance was 0.497 6; the maximum in-traspecific K2P distance of P. depressa was 0.005 2, while the minimum interspecific K2P distance was 0.519 1. The ITS2 secondary structure showed that P. asiatica and P. depressa can be differentiated obviously from its adulterants. Different samples of P. asiatica and P. depressa were gathered together and can be distinguished from its adulterants by NJ tree. It was concluded that the ITS2 sequence was able to identify original plant of Plantaginis Semen and its adulterants correctly. It provided a new method for the identification of original plant of Plantaginis Semen.
