Determination of Mannitol and Meglumine in Diterpene Lactones of Ginkgo Injection by HPLC-ELSD
10.11842/wst.2013.09.023
- VernacularTitle:HPLC-ELSD法测定注射用银杏二萜内酯中甘露醇和葡甲胺的含量
- Author:
Qinglong WU
;
Yongcheng SUN
;
Xuefeng LI
;
Wei WANG
;
Zhenzhong WANG
;
Yuan BI
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Diterpene Lactones of Ginkgo Injection;
mannitol;
meglumine;
HPLC-ELSD
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1985-1989
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to develop the method for determination of mannitol and meglumine in diterpene lactones of Ginkgo Injection. The HPLC-ELSD was used to determine mannitol and meglumine in diterpene lactones of Ginkgo Injection. The analysis was carried out on Waters XBridge Amide (4.6 mm í 150 mm, 3.5 μm) column. The mobile phase was the mixture of acetonitrile, and 50 mmol·mL-1 ammonium acetate with a linear gradient elu-tion at a flow rate of 1.0 mL·min-1. The column temperature wad maintained at 30℃. The operating conditions of the ELSD were a nebulizer nitrogen with the flow rate of 2.0 L·min-1 and an evaporator tube at the temperature of 90℃. The results showed that mannitol had a good linear relationship in a range of 1.9665 μg - 19.665 μg. The average recovery rate was 100.57% (RSD = 0.92%, n = 9). The meglumine showed a good linear relationship in a range of 0.4838 μg - 4.638 μg. The average recovery rate was 100.67% (RSD = 0.72%, n = 9). It was concluded that this method was simple and accurate with good reproducibility. It met with the requirement of the determination of the contents of mannitol and meglumine in diterpene lactones of Ginkgo Injection.
