Influence on Angiogenesis Factor and Expression of Its Receptor Protein of Estrogen-induced Uterine Fibroids Rat Model with Yi-Qi Xiao-Zheng Method
10.11842/wst.2013.09.033
- VernacularTitle:益气消法对雌激素诱导子宫肌瘤模型豚鼠血管内皮生长因子及其受体蛋白表达的影响
- Author:
Yizhu LU
;
Zhaohuan HUANG
;
Xiwei ZHANG
;
Zhuomin SONG
;
Na WANG
- Publication Type:Journal Article
- Keywords:
Uterine fibroids;
Y i-Qi Xiao-Zheng method;
microvessel density;
vascular endothelial growth factor;
vascular endothelial growth factor receptor
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):2047-2051
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe the influence on angiogenesis factor and expression of its receptor protein on estrogen-induced uterine fibroids rat models with Y i-Qi Xiao-Zheng (YQXZ) method, in order to explore the mechanism of this method in the inhibition of angiogenesis in the treatment of uterine fibroids. Castration and estro-gen-induced method were used in the establishment of guinea pig uterine fibroids rat model. And then, different doses of Chinese medicine of YQXZ method were given. The immunohistochemical method was used in the detection of microvessel density (MVD) and vascular endothelial growth factor (VEGF) protein expression of the uterine tissues. The RT-PCR method was used to detect the expression of VEGFR1 mRNA and VEGFR2 mRNA. Then, statistical analysis was given on indexes mentioned above. The results showed that compared with the normal control group, ex-pression of MVD, VEGF and VEGFR of uterine muscle tissues in the model group was significantly higher (P <0.01). Compared with the model group, Chinese medicine group of different doses showed different reducing. Among them, the high-dose group was the most obvious one. It showed that the high-dose Chinese medicine of YQXZ group was able to significantly inhibit the expression of MVD, VEGF and theirs receptors. It was concluded that YQXZ method can antagonize proliferation of the leiomyoma cell by inhibiting angiogenesis. Therefore, it may be one of the mechanisms of this method in the treatment of uterine fibroids.
