Optimization of Purification Technology for Sambucus williamsii Hance. by Macroporous Resin Adsorption Column Chromatography
10.11842/wst.2013.09.008
- VernacularTitle:大孔吸附柱色谱法优选接骨木的纯化工艺
- Author:
Hua HAN
;
Xin YIN
;
Bingyou YANG
;
Liu YANG
;
Yonggang XIA
;
Qiuhong WANG
;
Haixue KUANG
- Publication Type:Journal Article
- Keywords:
Sambucus williamsii Hance.;
morroniside;
macroporous resin adsorption column chromatography;
purification technology
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1907-1910
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to optimize the purification technology for Sambucus williamsii Hance. With the morroniside as a marker, the purification technology for Sambucus williamsii Hance was optimized by different types of macroporous resin. The results showed that the optimum purification technology was that, the extract of less than 1:250 (morroniside:resin) was adsorbed and the AB-8 resin was washed with distilled water, and then the morroni-side was eluted from the macroporous resin with 10% ethanol. And the content of the morroniside was more than 50%. It was concluded that the purification technology was simple, reliable, repeatable and suitable for industrial production.
