Experimental Study of Jinzhen Koufuye on Acute Lung Injury Induced by Lipopolysaccharide in Rats
10.11842/wst.2013.09.018
- VernacularTitle:金振口服液对脂多糖诱导大鼠急性肺损伤的实验研究
- Author:
Sujun LI
;
Zhaozhong HAN
;
Ping QIN
;
Jun ZHOU
;
Zhenzhong WANG
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Jinzhen Koufuye;
acute lung injury;
lipopolysaccharide;
inflammatory cytokines
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(9):1959-1965
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to explore effects of Jinzhen Koufuye (JZKFY) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Rats were randomly divided into the normal group, model group, positive control group, and three JZKFY groups with the drug dosage of 4.4, 2.2, 1.1 g·kg-1, respectively. Intragastric administration was given to animals for 7 consecutive days. One hour after the last administration, ALI model was induced by the injection of LPS (6 mg·kg-1), and then rats were killed at 16 h after saline (control) or LPS injection. Histological examinations were performed on the lungs. At the same time, the lung permeability index, activities of MPO, MDA and SOD in lung tissues, contents of TNF-α, IL-6 and IL-1β in serum were measured, respectively. The results showed that JZKFY of high-, medium-, or low-dosage can significantly reduce JZKFY lung permeability index. JZKFY of high- and medium-dosage can obviously regulate activities of MPO, MDA and SOD in lung tissues, re-duce contents of TNF-α, IL-6 and IL-1β in serum, as well as significantly improve lung tissue lesions in ALI. It was concluded that JZKFY might improve ALI induced by LPS through inhibiting lung vascular permeability, reduc-ing the lung neutrophil aggregation, improving the ability of antioxidative stress and lowering inflammatory response.
