Fingerprint and Multi-components Determination of Saponins in Sanjie-zhentong Capsule

VernacularTitle:散结镇痛胶囊中皂苷类成分的指纹图谱研究和多指标成分定量测定
Author: Jianping QIN ; Jianxiong WU ; Jiachun LI ; Baolai CHEN ; Hongmei WEN ; Wei XIAO
Publication Type:Journal Article
Keywords: Sanjie-zhentong Capsule; HPLC; multi-components; fingerprint
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(9):1980-1984
CountryChina
Language:Chinese
Abstract: This study was aimed to establish a HPLC fingerprint of saponins in Sanjie-zhentong Capsule in order to make a quantitative analysis of the quality of Sanjie-zhentong Capsule. The Waters Symmetry ShieldTM RP18 (4.6 mmí 250 mm, 5 μm) column was used with a mobile phase of acetonitrile-water gradient elution. The flow rate was 1.2 mL/min. The column temperature was 30℃. The detection wavelength was 203 nm. The results showed that the fingerprint chromatography included 9 mutual peaks. The similarity among batches was more than 0.95. Compared with reference substance, five characteristic components were recognized. The five components are notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 and ginsenoside Rd. It was concluded that this method was rapid, simple and accurate and can be used as one of the effective methods for the quality control of Sanjie-zhen-tong Capsule.