Analysis on brain targeting of scopolamine following intranasal administration by using solid phase extraction and LC/MS assay
10.3969/j.issn.1671-8348.2013.36.017
- VernacularTitle:固相萃取与液质联用法分析东莨菪碱经鼻腔给药的脑靶向性
- Author:
Ping LI
;
Huafen WANG
;
Weifeng SHI
;
Lanlan LIU
;
Wei LIU
- Publication Type:Journal Article
- Keywords:
scopolamine;
administration,intranasal;
solid phase extraction;
LC/MS assay
- From:
Chongqing Medicine
2013;(36):4405-4407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of scopolamine into brain following intranasal .Methods Adult Wistar rats were divided into two groups ,and administrated with scopolamine 0 .3 mg via intraperitoneal or intranasal respectively .Two groups of blood concentration and brain concentration of scopolamine were determined by solid phase extraction (SPE) and LC/MS assay at the time point of 5 ,10 ,20 ,30 ,60 ,120 ,240 ,480 min ,and their kinetic parameters were calculated and compared .Results Scopol-amine bioavailability of intranasal administration was 65% ,and the maximum concentration of scopolamine in brain following in-tranasal administration was much higher than that following intraperitoneal administration .Comparing the ratio of AUC in brain and in plasma ,ratio of intranasal administration is bigger than that of intraperitoneal administration significantly (P<0 .05) .Con-clusion It was concluded that scopolamine′s intranasal administration induced the agent targeting distribution into the brain .
