The effect of over-expression of C/EBPαon HSC-T6 cell actived by PAR1

VernacularTitle:上调C／EBPα对 PAR1激活的 HSC-T6细胞的影响
Author: Yingchun LIU ; Xiaohong GU ; Yundong ZHANG ; Xuwei DUAN
Publication Type:Journal Article
Keywords: CCAAT enhancer binding protein α; receptor,RAP-1; hepatic stellate cells
From: Chongqing Medicine 2013;(36):4424-4426
CountryChina
Language:Chinese
Abstract: Objective To explore the over-expression of the CCAAT enhancer binding proteinα(C/EBPα)in hepatic stellate cells (HSC-T6) which activated by protease-activated receptor 1(PAR1) ,to explore its possible relationship with cell proliferation ,cell activation and collagen secretion of HSC-T6 cell .Methods HSC-T6 cells were divided into 4 groups ,control group ,SFLLR group;vehicle plasmid+SFLLR group ,C/EBPαplasmid+SFLLR group .The eukaryotic vector harboring the full length of C/EBPαcD-NA was transfected into PAR1 activated HSC-T6 cell ,then Western blot was applied to detect the expression of α-smooth muscle actin(α-SMA) and collagen I and evaluate the effect of transfection on proliferation of HSC-T6 by MTT .Results The expression of α-SMA and collagen I in C/EBPαplasmid+SFLLR group were dramatically decreased compared with other 3 groups ,as well as proliferation after 48 h(P<0 .05) .Conclusion Over-expression of C/EBPαgene by transfection had inhibitory influence on prolif-eration ,activation and collagen secretion of HSC-T6 which were activated by PAR1 .