The effect of different adjuvants and challenge methods on asthma mice′s lung inflammation
10.3969/j.issn.1671-8348.2013.36.027
- VernacularTitle:不同佐剂和激发方式对哮喘小鼠肺部炎性反应的影响
- Author:
Wenqing ZHONG
;
Jianxin TAN
- Publication Type:Journal Article
- Keywords:
asthma;
aluminium hydroxide;
aluminum potassium sulfate dodecahydrate;
bronchoalveolar lavage fluid
- From:
Chongqing Medicine
2013;(36):4433-4435
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different adjuvants and challengemethods on asthma mice′s lung inflammation . Methods BALB/c mice were sensitized with ovalbumin in aluminium hydroxide or alum adjuvant .After 7 days challenges ,choose the proper adjuvant with intranasal or atomized to prepare mice asthma model .Count and classify the white blood cells (WBC) in bronchoalveolar lavage fluid(BALF) ,observe the peribronchial and perivascular inflammation .Results Compare the WBC and eos-nophils in BALF ,the alum group are higher than aluminum hydroxide group(P<0 .05) ,5 and 7 days′atomized groups are highter than 3 days′intranasal group(P<0 .05) ,5 and 7 days′atomized groups have showed no significant difference (P>0 .05) .10 days after the last challenge ,the inflammation fade sharply ,and the corresponding result occured in the lung inflammation .Conclusion In establishing asthma model ,alum is better than aluminum hydroxide ,atomization is better than intranasal .Inflammation will fade after stop atomization a period of time .
