Toxicity and Anti-inflammatory Effect of Total Saponins from Panax japonicus by Different Extraction Technology

VernacularTitle:不同工艺提取的竹节参皂苷粗提物毒性及其抗炎作用初步研究
Author: Yanwen DAI ; Ding YUAN ; Haibin ZHANG ; Jingzhi WAN ; Zhiwei SUN ; Chiqing CHEN ; Changcheng ZHANG ; Ting WANG
Publication Type:Journal Article
Keywords: saponins; extraction process; saponins from Panax japonicus; anti-inflammatory; cell activity
From: Chinese Journal of Information on Traditional Chinese Medicine 2014;(1):50-53
CountryChina
Language:Chinese
Abstract: Objective To compare the toxicity and anti-inflammatory effect of total saponins from Panax japonicus by different extraction technology. Methods The total saponins of sample 1, sample 2, sample 3, sample 4, sample 5 and sample 6 was prepared respectively by different process, and RAW264.7 cells were treated with the samples of different concentration. Then cells morphology was observed under microscope, thiazolyl blue (MTT) method was used to detect cell activity, the nitric oxide (NO) release of RAW264.7 cells was detected with NO kit. Results The cell toxicity of different samples from low to high was as follows:sample 4sample 5>sample 2>sample 6>sample 1>sample 3. Conclusion Among these six different kinds of extraction process of total saponins from Panax japonicus, the total saponins extract by foam fractionation has not only the minimal toxicity, but also the best primary anti-inflammatory effect.