Effect of Different Purity Galangin on Melanocyte Proliferation and Melanin Synthesis of A375-HaCaT Co-culture System
10.3969/j.issn.1005-5304.2014.01.018
- VernacularTitle:不同纯度高良姜素对A375-HaCaT共培养模型中黑素细胞增殖及黑素合成的影响
- Author:
Xiaoming PENG
;
Shixia HUO
;
Pingping ZHAO
;
Li GAO
;
Ming YAN
- Publication Type:Journal Article
- Keywords:
Galangin;
melanoma cells;
keratinocytes;
co-culture model;
cell proliferation;
melanin;
ET
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2014;(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Galangin of different purity on melanocyte proliferation and melanin synthesis of A375-HaCaT co-culture system. Methods The A375-HaCaT co-culture system was established with Transwell technology, and 0.1, 0.5, 1.0, 5.0, 10 μg/mL Galangin of the purity of 70%and 90%was used to act on the co-culture system. Cell proliferation, melanin content and tyrosinase of A375 were measured by MTT assay, NaOH lysis assay and L-DOPA oxidation assay respectively. The cytokines such as ET-1 and SCF in HaCaT were detected by ELISA. Results A375 cells in co-culture system grew well, and 0.1, 0.5, 1.0, 5.0, 10 μg/mL Galangin of the purity of 70% and 90% had up-regulation effect on cell proliferation, melanin content and tyrosinase of A375. Moreover, Galangin could increase the expression level of ET-1 and SCF of HaCaT at more than 0.5 μg/mL, and the effect of Galangin of 70% purity was better than 90% purity. Conclusion Galangin could promote the cell proliferation, melanin content and tyrosinase of A375, and mechanism of the pathways is probably related to the up-regulation on the expression of ET-1 and SCF of HaCaT.
